{"id":17884,"date":"2023-08-17T12:02:42","date_gmt":"2023-08-17T04:02:42","guid":{"rendered":"https:\/\/www.nikyang.com\/?p=17884"},"modified":"2023-08-17T12:03:04","modified_gmt":"2023-08-17T04:03:04","slug":"dbs-animal_antibodies-detection-case","status":"publish","type":"post","link":"https:\/\/www.nikyang.com\/bio-sample-prep\/dbs-animal_antibodies-detection-case\/","title":{"rendered":"DBS \u5e72\u8840\u6591\u5e94\u7528\u7279\u96c6 4: \u725b\u5b62\u75b9\u75c5\u6bd2\u6297\u4f53\u7684\u68c0\u6d4b"},"content":{"rendered":"\t\t

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\u725b\u9f3b\u6c14\u7ba1\u708e\u53ca\u5176\u8bca\u65ad<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u725b\u9f3b\u6c14\u7ba1\u708e (bovine rhinotracheitis, IBR) \u662f\u4e00\u79cd\u725b\u7684\u6d41\u884c\u6027\u75be\u75c5\uff0c\u60a3\u75c5\u4f1a\u5f15\u8d77\u725b\u7684\u4ea7\u91cf\u4e0b\u964d\u3001\u9ad8\u989d\u7684\u6cbb\u7597\u8d39\u7528\u548c\u8d38\u6613\u9650\u5236\uff0c\u56e0\u6b64\u4f1a\u5728\u4e16\u754c\u8303\u56f4\u5185\u9020\u6210\u5de8\u5927\u7684\u7ecf\u6d4e\u635f\u5931\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t $\"\u725b\u9f3b\u6c14\u7ba1\u708e\"$ \t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u725b\u5b62\u75b9\u75c5\u6bd2 BoHV-1 \u662f\u4f20\u67d3\u6027\u725b\u9f3b\u6c14\u7ba1\u708e (IBR) \u7684\u75c5\u539f\u4f53\uff0c\u800c BoHV-1 \u6297\u4f53\u5219\u662f\u8bca\u65ad\u725b\u9f3b\u6c14\u7ba1\u708e\u7684\u751f\u7269\u6807\u8bb0\u7269\u3002\u5bf9\u725b\u8fdb\u884c\u5b9a\u671f\u7684 BoHV-1 \u6297\u4f53\u76d1\u6d4b\u53ef\u4ee5\u5728\u75c5\u725b\u60a3\u75c5\u521d\u671f\u8fdb\u884c\u4e2a\u4f53\u8bc6\u522b\u4e0e\u9694\u79bb\uff0c\u6709\u6548\u5730\u964d\u4f4e\u725b\u9f3b\u6c14\u7ba1\u708e\u5728\u725b\u7fa4\u4e2d\u5927\u89c4\u6a21\u4f20\u64ad\u7684\u98ce\u9669\uff0c\u56e0\u6b64\u5bf9\u4fdd\u969c\u755c\u7267\u4e1a\u6536\u76ca\u5177\u6709\u91cd\u8981\u7684\u4ef7\u503c\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u5e72\u8840\u6591:\n\n\u65b9\u4fbf\u7684\u8840\u6837\u91c7\u96c6<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u76ee\u524d BoHV-1 \u6297\u4f53\u76d1\u6d4b\u91c7\u7528\u7684\u662f\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a (Enzyme-linked immunosorbent assay, ELISA)\uff0c\u9700\u8981\u7531\u517d\u533b\u62bd\u53d6\u725b\u7684\u9759\u8109\u8840\u6db2\uff0c\u79bb\u5fc3\u5206\u79bb\u51fa\u8840\u6d46\uff0c\u518d\u8fdb\u884c\u68c0\u6d4b\u3002\u9759\u8109\u53d6\u8840\u7684\u53d6\u8840\u91cf\u5927\uff0c\u4e14\u8fc7\u7a0b\u6bd4\u8f83\u590d\u6742\uff0c\u9700\u8981\u517d\u533b\u5177\u6709\u4e30\u5bcc\u7684\u7ecf\u9a8c\u3002\u53d6\u8840\u8fc7\u7a0b\u4e2d\uff0c\u52a8\u7269\u5f80\u5f80\u4f1a\u51fa\u73b0\u62b5\u89e6\u60c5\u7eea\uff0c\u6709\u65f6\u751a\u81f3\u4f1a\u53d7\u5230\u60ca\u5413\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t $\"\"$ \t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t $\"\"$ \t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u5e72\u8840\u6591 (Dried blood spots, DBS) \u662f\u4e00\u79cd\u65b0\u578b\u7684\u8840\u6db2\u91c7\u96c6\u548c\u50a8\u5b58\u65b9\u6cd5\u3002\u5b83\u5c06\u5fae\u5347\u7ea7\u672b\u68a2\u8840\u91c7\u96c6\u5230\u6837\u54c1\u5361\u4e0a\uff0c\u5e72\u71e5\u540e\u5f62\u6210\u6591\u70b9\u3002\u8be5\u65b9\u6cd5\u91c7\u6837\u8fc7\u7a0b\u7b80\u5355\u65b9\u4fbf\uff0c\u9972\u4e3b\u53ef\u81ea\u884c\u64cd\u4f5c\uff1b\u4fb5\u5165\u6027\u5c0f\uff0c\u7279\u522b\u9002\u5408\u5bf9\u5c0f\u578b\u52a8\u7269 \/ \u5ba0\u7269\u548c\u5b9e\u9a8c\u52a8\u7269\u8fdb\u884c\u8840\u6837\u91c7\u96c6\u3002<\/p>
\u76f8\u6bd4\u4e8e\u6db2\u6001\u8840\uff0c\u56fa\u6001\u7684\u5e72\u8840\u6591\u5177\u5907\u66f4\u597d\u7684\u7a33\u5b9a\u6027\u548c\u751f\u7269\u5b89\u5168\u6027\uff0c\u53ef\u5b9e\u73b0\u6837\u54c1\u7684\u5e38\u6e29\u8fd0\u8f93\uff0c\u66f4\u53ef\u4ee5\u4ee5\u66f4\u6e29\u548c\u7684\u6761\u4ef6\u50a8\u5b58\u6837\u54c1\uff0c\u6784\u5efa\u751f\u7269\u6837\u672c\u5e93\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u7b80\u5355\u8840\u6837\u91c7\u96c6\u6b65\u9aa4<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t $\"\u9972\u4e3b\u81ea\u884c\u91c7\u6837\"$ \t\t\t\t\t\t\t\t\t\t\t
1.\u9972\u4e3b\u81ea\u884c\u91c7\u6837<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t $\"\"$ \t\t\t\t\t\t\t\t\t\t\t
2.\u90ae\u5bc4\u6837\u54c1<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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3.\u68c0\u6d4b<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t $\"\"$ \t\t\t\t\t\t\t\t\t\t\t
4.\u8bca\u65ad\u6cbb\u7597<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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DBS \u5e72\u8840\u6591\u5e94\u7528\u6848\u4f8b:
\n\n\n\u725b\u5b62\u75b9\u75c5\u6bd2\u6297\u4f53\u7684\u68c0\u6d4b<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u7814\u7a76\u9996\u6b21\u5c06 DBS \u6280\u672f\u5e94\u7528\u4e8e BoHV-1 \u6297\u4f53\u7684\u68c0\u6d4b\u4e2d\uff0c\u4ece\u6280\u672f\u4e0a\u8bc1\u660e\u4e86\u5176\u5e94\u7528\u53ef\u884c\u6027\u3002BoHV-1 \u9633\u6027\u548c\u9634\u6027\u7684\u5e72\u8840\u6591\u6837\u672c\u901a\u8fc7\u5168\u81ea\u52a8\u5e72\u8840\u6591\u8403\u53d6\u5e73\u53f0 DBS-MS 500 Hct \u76f4\u63a5\u8403\u53d6\u6536\u96c6\uff0c\u518d\u8fdb\u884c ELISA \u6d4b\u8bd5\uff0c\u68c0\u6d4b BoHV-1 \u6297\u4f53\u3002<\/p>
\u4e0e\u5e72\u8840\u6591\u6837\u54c1\u5bf9\u5e94\u7684\u8840\u6d46\u6837\u672c (BoHV-1 \u9633\u6027\u548c\u9634\u6027) \u4e5f\u91c7\u7528\u76f8\u540c\u7684\u68c0\u6d4b\u65b9\u5f0f\u8fdb\u884c\u68c0\u6d4b\uff0c\u7528\u4e8e\u5bf9\u6bd4\u5e72\u8840\u6591\u6837\u672c\u4e0e\u4f20\u7edf\u8840\u6d46\u6837\u672c\u7684\u5f02\u540c\u3002\u53e6\u5916\uff0c\u6b64\u9879\u7814\u7a76\u8fd8\u63a2\u8ba8\u4e86\u5e72\u8840\u6591\u7684\u50a8\u5b58\u6761\u4ef6\u4e0e\u65f6\u95f4\u5bf9 BoHV-1 \u6297\u4f53\u68c0\u6d4b\u7ed3\u679c\u7684\u5f71\u54cd\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u68c0\u6d4b\u76ee\u6807<\/strong><\/h5><\/section><\/section><\/section><\/section><\/section>
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BoHV-1 \u6297\u4f53<\/p><\/section><\/section><\/section>
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Ahlstrom AutoCollect^{TM\u00a0<\/sup><\/p><\/section><\/section><\/section>}
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\u6837\u672c\u8fd0\u8f93\u6761\u4ef6<\/strong><\/h5><\/section><\/section><\/section><\/section><\/section>
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\u5ba4\u6e29\u4e09\u5929\u3001-20\u00b0C \u56db\u4e2a\u6708\u3001\u4fdd\u5b58\u56db\u5468 (\u542b 4 \u4e2a\u4e0d\u540c\u6761\u4ef6: 4\u00b0C\u3001\u5ba4\u6e29\u300137\u00b0C \u53ca\u4e00\u5c0f\u65f6\u7d2b\u5916\u5149\u7167\u540e\u5ba4\u6e29)<\/p><\/section><\/section><\/section>
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\u63d0\u53d6\u65b9\u6cd5<\/strong><\/h5><\/section><\/section><\/section><\/section><\/section>
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Epitope ELISA kit \u7f13\u51b2\u6db2\u76f4\u63a5\u63d0\u53d6<\/p><\/section><\/section><\/section>
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\u68c0\u6d4b\u65b9\u6cd5<\/strong><\/h5><\/section><\/section><\/section><\/section><\/section>
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ELISA\u3001IDEXX IBR gB X3 Ab Test kit<\/p><\/section><\/section><\/section>
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\u7ed3\u679c<\/strong><\/h5><\/section><\/section>
\u5e72\u8840\u6591\u6837\u672c\u7684\u68c0\u6d4b\u7ed3\u679c\u4e0e\u4f20\u7edf\u8840\u6d46\u6837\u672c\u7ed3\u679c\u5b8c\u5168\u4e00\u81f4\u3002<\/p>
\u53e6\u5916\uff0c\u5e72\u8840\u6591\u6837\u672c\u5728\u5ba4\u6e29\u4e0b\u4fdd\u5b58 3 \u5929\u6216\u5728 -20\u00b0C \u4fdd\u5b58 4 \u4e2a\u6708\uff0c\u90fd\u80fd\u5f97\u5230\u51c6\u786e\u7684\u68c0\u6d4b\u7ed3\u679c\uff0c\u56e0\u6b64\u5176\u9ad8\u7a33\u5b9a\u6027\u53ef\u4ee5\u5141\u8bb8\u5e72\u8840\u6591\u6837\u672c\u7684\u5ba4\u6e29\u8fd0\u8f93\u53ca\u957f\u671f\u4fdd\u5b58\uff0c\u7279\u522b\u9002\u5408\u7528\u4e8e\u76f8\u5173\u75be\u75c5\u7684\u5927\u89c4\u6a21\u7b5b\u67e5\u3002<\/p><\/section>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u56fe 1. \u725b\u5e72\u8840\u6591\u91c7\u96c6\u548c \u5168\u81ea\u52a8\u6837\u54c1\u8403\u53d6\u6d41\u7a0b\u56fe\u3002<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u56fe 2. ELISA \u6297\u4f53\u6548\u4ef7\u7ed3\u679c\u3002<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u4ece\u5de6\u81f3\u53f3\u7684\u4e94\u4e2a\u6761\u4ef6\u5206\u522b\u4e3a\u5ba4\u6e29\u4fdd\u5b58 3 \u5929\u7684\u9633\u6027\u5e72\u8840\u6591\u3001-20\u00b0C \u4fdd\u5b58 4 \u4e2a\u6708\u7684\u9633\u6027\u5e72\u8840\u6591\u6837\u672c\u3001\u9633\u6027\u8840\u6d46\u6837\u672c\u3001\u9634\u6027\u5e72\u8840\u6591\u6837\u672c\u548c\u9634\u6027\u8840\u6d46\u6837\u672c\u3002\u6297\u4f53\u6548\u4ef7\u8bf4\u660e: >55% \u8ba4\u5b9a\u4e3a\u9633\u6027\uff0c<45% \u8ba4\u5b9a\u4e3a\u9634\u6027\uff0c45% – 55% \u4e4b\u95f4\u4e3a\u6000\u7591\u6837\u672c\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u53c2\u8003\u6587\u732e:<\/em><\/p>
Lechmann, J.; Gehrig, O.; Luginb\u00fchl, M.; Fraefel, C.; Gaugler, S.\u00a0Fully automated dried blood spot sample handling and extraction for BoHV-1 antibody testing by ELISA. J. Virol. Methods 2022, 310, 114626.<\/em><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u914d\u5408\u5e72\u8840\u6591\u52a0\u5feb\u8fdb\u884c\u751f\u7269\u6807\u8bb0\u7269\u7684\u7814\u7a76\uff0c\u529b\u626c\u63a8\u51fa CAMAG \u5168\u81ea\u52a8\u5e72\u8840\u6591\u8403\u53d6\u7cfb\u7edf DBS-MS 500\uff0c\u4ece\u81ea\u52a8\u5316\u65b9\u5411\u5bf9\u4f20\u7edf\u5e72\u8840\u6591\u6280\u672f\u8fdb\u884c\u7684\u4e00\u6b21\u5168\u9762\u5347\u7ea7\uff0c\u53ef\u5b9e\u73b0\u5168\u81ea\u52a8\u3001\u5feb\u901f\u3001\u65e0\u4eba\u503c\u5b88\u7684\u5e72\u8840\u6591\u6837\u54c1\u5236\u5907\uff0c\u5e76\u53ef\u7ed3\u5408\u8d28\u8c31\u3001PCR\u3001NGS \u6d4b\u5e8f\u53ca ELISA \u7b49\u68c0\u6d4b\u6280\u672f\uff0c\u5e94\u7528\u4e8e\u4ee5\u4e0b\u9886\u57df\uff1a<\/p>
\u5065\u5eb7\u751f\u7269\u6807\u8bb0\u7269\u68c0\u6d4b<\/h6><\/li>
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\n\t\t\t\t\t\t\t\t\t\t $\"\"$ \t\t\t\t\t\t\t\t\t\t\t
\u5168\u81ea\u52a8\u5e72\u8840\u6591 \u8403\u53d6\u7cfb\u7edf<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"
\u725b\u9f3b\u6c14\u7ba1\u708e\u53ca\u5176\u8bca\u65ad \u725b\u9f3b\u6c14\u7ba1\u708e (bovine rhinotracheitis, IBR) \u662f\u4e00\u79cd\u725b\u7684\u6d41\u884c\u6027\u75be\u75c5\uff0c\u60a3\u75c5\u4f1a\u5f15\u8d77\u725b\u7684\u4ea7\u91cf\u4e0b\u964d\u3001\u9ad8\u989d\u7684\u6cbb\u7597\u8d39\u7528\u548c\u8d38\u6613\u9650\u5236\uff0c\u56e0\u6b64\u4f1a\u5728\u4e16\u754c\u8303\u56f4\u5185\u9020\u6210\u5de8\u5927\u7684\u7ecf\u6d4e\u635f\u5931\u3002 \u725b\u5b62\u75b9\u75c5\u6bd2 BoHV-1 \u662f\u4f20\u67d3\u6027\u725b\u9f3b\u6c14\u7ba1\u708e (IBR) \u7684\u75c5\u539f\u4f53\uff0c\u800c BoHV-1 \u6297\u4f53\u5219\u662f\u8bca\u65ad\u725b\u9f3b\u6c14\u7ba1\u708e\u7684\u751f\u7269\u6807\u8bb0\u7269\u3002\u5bf9\u725b\u8fdb\u884c\u5b9a\u671f\u7684 BoHV-1 \u6297\u4f53\u76d1\u6d4b\u53ef\u4ee5\u5728\u75c5\u725b\u60a3\u75c5\u521d\u671f\u8fdb\u884c\u4e2a\u4f53\u8bc6\u522b\u4e0e\u9694\u79bb\uff0c\u6709\u6548\u5730\u964d\u4f4e\u725b\u9f3b\u6c14\u7ba1\u708e\u5728\u725b\u7fa4\u4e2d\u5927\u89c4\u6a21\u4f20\u64ad\u7684\u98ce\u9669\uff0c\u56e0\u6b64\u5bf9\u4fdd\u969c\u755c\u7267\u4e1a\u6536\u76ca\u5177\u6709\u91cd\u8981\u7684\u4ef7\u503c\u3002 \u5e72\u8840\u6591: \u65b9\u4fbf\u7684\u8840\u6837\u91c7\u96c6 \u76ee\u524d BoHV-1 \u6297\u4f53\u76d1\u6d4b\u91c7\u7528\u7684\u662f\u9176\u8054\u514d\u75ab\u5438\u9644\u6d4b\u5b9a (Enzyme-linked immunosorbent assay, ELISA)\uff0c\u9700\u8981\u7531\u517d\u533b\u62bd\u53d6\u725b\u7684\u9759\u8109\u8840\u6db2\uff0c\u79bb\u5fc3\u5206\u79bb\u51fa\u8840\u6d46\uff0c\u518d\u8fdb\u884c\u68c0\u6d4b\u3002\u9759\u8109\u53d6\u8840\u7684\u53d6\u8840\u91cf\u5927\uff0c\u4e14\u8fc7\u7a0b\u6bd4\u8f83\u590d\u6742\uff0c\u9700\u8981\u517d\u533b\u5177\u6709\u4e30\u5bcc\u7684\u7ecf\u9a8c\u3002\u53d6\u8840\u8fc7\u7a0b\u4e2d\uff0c\u52a8\u7269\u5f80\u5f80\u4f1a\u51fa\u73b0\u62b5\u89e6\u60c5\u7eea\uff0c\u6709\u65f6\u751a\u81f3\u4f1a\u53d7\u5230\u60ca\u5413\u3002 \u5e72\u8840\u6591 (Dried blood spots, DBS) \u662f\u4e00\u79cd\u65b0\u578b\u7684\u8840\u6db2\u91c7\u96c6\u548c\u50a8\u5b58\u65b9\u6cd5\u3002\u5b83\u5c06\u5fae\u5347\u7ea7\u672b\u68a2\u8840\u91c7\u96c6\u5230\u6837\u54c1\u5361\u4e0a\uff0c\u5e72\u71e5\u540e\u5f62\u6210\u6591\u70b9\u3002\u8be5\u65b9\u6cd5\u91c7\u6837\u8fc7\u7a0b\u7b80\u5355\u65b9\u4fbf\uff0c\u9972\u4e3b\u53ef\u81ea\u884c\u64cd\u4f5c\uff1b\u4fb5\u5165\u6027\u5c0f\uff0c\u7279\u522b\u9002\u5408\u5bf9\u5c0f\u578b\u52a8\u7269 \/ \u5ba0\u7269\u548c\u5b9e\u9a8c\u52a8\u7269\u8fdb\u884c\u8840\u6837\u91c7\u96c6\u3002 \u76f8\u6bd4\u4e8e\u6db2\u6001\u8840\uff0c\u56fa\u6001\u7684\u5e72\u8840\u6591\u5177\u5907\u66f4\u597d\u7684\u7a33\u5b9a\u6027\u548c\u751f\u7269\u5b89\u5168\u6027\uff0c\u53ef\u5b9e\u73b0\u6837\u54c1\u7684\u5e38\u6e29\u8fd0\u8f93\uff0c\u66f4\u53ef\u4ee5\u4ee5\u66f4\u6e29\u548c\u7684\u6761\u4ef6\u50a8\u5b58\u6837\u54c1\uff0c\u6784\u5efa\u751f\u7269\u6837\u672c\u5e93\u3002 \u7b80\u5355\u8840\u6837\u91c7\u96c6\u6b65\u9aa4 1.\u9972\u4e3b\u81ea\u884c\u91c7\u6837 2.\u90ae\u5bc4\u6837\u54c1 3.\u68c0\u6d4b 4.\u8bca\u65ad\u6cbb\u7597 DBS \u5e72\u8840\u6591\u5e94\u7528\u6848\u4f8b: \u725b\u5b62\u75b9\u75c5\u6bd2\u6297\u4f53\u7684\u68c0\u6d4b \u7814\u7a76\u9996\u6b21\u5c06 DBS \u6280\u672f\u5e94\u7528\u4e8e BoHV-1 \u6297\u4f53\u7684\u68c0\u6d4b\u4e2d\uff0c\u4ece\u6280\u672f\u4e0a\u8bc1\u660e\u4e86\u5176\u5e94\u7528\u53ef\u884c\u6027\u3002BoHV-1 \u9633\u6027\u548c\u9634\u6027\u7684\u5e72\u8840\u6591\u6837\u672c\u901a\u8fc7\u5168\u81ea\u52a8\u5e72\u8840\u6591\u8403\u53d6\u5e73\u53f0 DBS-MS 500 Hct \u76f4\u63a5\u8403\u53d6\u6536\u96c6\uff0c\u518d\u8fdb\u884c ELISA \u6d4b\u8bd5\uff0c\u68c0\u6d4b BoHV-1 \u6297\u4f53\u3002 […]<\/p>\n","protected":false},"author":17,"featured_media":17885,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[151,186],"tags":[97,131],"_links":{"self":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts\/17884"}],"collection":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/comments?post=17884"}],"version-history":[{"count":5,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts\/17884\/revisions"}],"predecessor-version":[{"id":17896,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts\/17884\/revisions\/17896"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/media\/17885"}],"wp:attachment":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/media?parent=17884"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/categories?post=17884"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/tags?post=17884"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}