{"id":17446,"date":"2023-05-04T15:16:18","date_gmt":"2023-05-04T07:16:18","guid":{"rendered":"https:\/\/www.nikyang.com\/?p=17446"},"modified":"2023-05-04T16:31:10","modified_gmt":"2023-05-04T08:31:10","slug":"hptlc-protein-case","status":"publish","type":"post","link":"https:\/\/www.nikyang.com\/news\/hptlc-protein-case\/","title":{"rendered":"HPTLC-MS \u5e94\u7528\u4e8e\u86cb\u767d\u8d28\u7684\u80f0\u86cb\u767d\u9176\u6c34\u89e3\u7269\u7684\u5206\u6790\u7814\u7a76"},"content":{"rendered":"\t\t
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\u8fd1\u5e74\u6765\uff0c\u968f\u7740\u8584\u5c42\u677f\u56fa\u5b9a\u76f8\u6d82\u5c42\u591a\u6837\u5316\u7684\u53d1\u5c55\uff0c\u9ad8\u6548\u8584\u5c42\u8272\u8c31\u6280\u672f\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u8bf8\u591a\u9886\u57df\uff0c\u5c24\u5176\u662f TLC-MS \u7684\u8054\u7528\u548c\u5546\u4e1a\u5316\u8bbe\u5907\u7684\u65e5\u8d8b\u6210\u719f\uff0c\u4e3a\u751f\u7269\u5206\u6790\u7814\u7a76\u63d0\u4f9b\u4e86\u65b0\u7684\u601d\u8def\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\u7814\u7a76<\/strong>\u76ee\u7684<\/strong><\/h4><\/section>
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\u672c\u7814\u7a76\u5c06\u808c\u7ea2\u86cb\u767d\u3001\u7ec6\u80de\u8272\u7d20 C\u3001\u03b2-\u916a\u86cb\u767d\u548c\u725b\u8840\u6e05\u86cb\u767d (BSA) \u7b49\u86cb\u767d\u8d28\u7684\u80f0\u86cb\u767d\u9176\u5206\u89e3\u7269\u4e2d\u7684\u80bd\u901a\u8fc7\u9ad8\u6548\u8584\u5c42\u8272\u8c31\u6cd5\u8fdb\u884c\u5206\u79bb\uff0c\u7136\u540e\u901a\u8fc7\u4e0e ESI-MS \u5076\u8054\u5728\u8584\u5c42\u677f\u4e0a\u539f\u4f4d\u5206\u6790\uff0c\u786e\u5b9a\u5e8f\u5217\u8986\u76d6\u7387\u5e76\u4e0e\u6db2\u76f8\u68c0\u6d4b\u7ed3\u679c\u8fdb\u884c\u6bd4\u8f83\u3002<\/p>

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\u4f7f\u7528\u4e00\u7ef4 HPTLC \u6700\u591a\u5206\u79bb\u5f97\u5230 20 \u4e2a\u6761\u5e26\uff0c\u83b7\u5f97\u975e\u5e38\u7d27\u51d1\u7684\u8584\u5c42\u8272\u8c31\u533a\uff0c\u8fd9\u5927\u7ea6\u662f\u901a\u8fc7\u80f0\u86cb\u767d\u9176\u6d88\u5316\u86cb\u767d\u8d28\u6837\u54c1\u83b7\u5f97\u7684\u80bd\u7684\u6570\u91cf (\u4f8b\u5982\u7ec6\u80de\u8272\u7d20 C \u7531 104 \u4e2a\u6c28\u57fa\u9178\u7ec4\u6210\uff0c\u7ecf\u8fc7\u80f0\u86cb\u767d\u9176\u6d88\u5316\u540e\u5f62\u6210 17 \u4e2a\u80bd\uff09\u3002<\/p><\/section>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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HPTLC \u4e00\u7ef4\u5206\u79bb\u8272\u8c31\u56fe\uff1a \u56fe 1. \u8367\u5149\u80fa\u663e\u8272-366nm\uff1b\u56fe 2. \u831a\u4e09\u916e\u663e\u8272-\u767d\u5149 [\u6837\u54c1\u4ece\u5de6\u81f3\u53f3\u5206\u522b\u4e3a\uff1a\u808c\u7ea2\u86cb\u767d\u3001\u7ec6\u80de\u8272\u7d20 C\u3001\u03b2-\u916a\u86cb\u767d\u548c\u725b\u8840\u6e05\u86cb\u767d (BSA)]<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u518d\u8fdb\u4e00\u6b65\u8584\u5c42\u5c55\u5f00\uff0c\u901a\u8fc7 HPTLC \u4e8c\u7ef4\u5206\u79bb\uff0c\u53ef\u4ee5\u589e\u52a0\u88ab\u5206\u79bb\u7269\u8d28\u7684\u6570\u91cf\u3002\u7531\u4e0b\u56fe\u53ef\u77e5\u4e00\u7ef4\u5206\u79bb\u8272\u8c31\u53ef\u89c1 11 \u4e2a\u5206\u79bb\u6761\u5e26\uff0c\u4e8c\u7ef4\u5206\u79bb\u8272\u8c31\u53ef\u89c1 17 \u4e2a\u5206\u79bb\u6591\u70b9\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\u56fe 3. HPTLC \u4e8c\u7ef4\u5206\u79bb\u8272\u8c31\u56fe\uff1a\u7ec6\u80de\u8272\u7d20 C<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u8868 1. HPTLC \/ DESI-MS \u5206\u6790\u540e\u83b7\u5f97\u7684\u7ec6\u80de\u8272\u7d20 C \u7684\u5e8f\u5217\u8986\u76d6\u7387<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u7531\u6b64\u53ef\u89c1\uff0cHPTLC-MS \u975e\u5e38\u9002\u5408\u5206\u6790\u7b80\u5355\u548c\u4e2d\u7b49\u590d\u6742\u7684\u80bd\u6df7\u5408\u7269\u3002HPTLC \/ MS \u8054\u7528\u7684\u4e00\u4e2a\u7279\u70b9\u662f\u5206\u79bb\u548c\u68c0\u6d4b\u53ef\u4ee5\u5404\u81ea\u72ec\u7acb\u8fdb\u884c\uff0c\u56e0\u6b64\u57fa\u4e8e\u8584\u5c42\u5f00\u653e\u6027\u8272\u8c31\u7684\u7279\u70b9\u53ef\u4ee5\u7075\u6d3b\u4f18\u5316\u5206\u79bb\u548c\u884d\u751f\u5316\u6761\u4ef6\uff0c\u4f7f\u5206\u79bb\u7ed3\u679c\u5f97\u5230\u66f4\u591a\u53ef\u80fd\u3002\u540c\u65f6\u8584\u5c42\u677f\u5747\u4e3a\u4e00\u6b21\u6027\u4f7f\u7528\uff0c\u53ef\u4e3a\u5404\u79cd\u6761\u4ef6\u4f18\u5316\u8bbe\u8ba1\u5e26\u6765\u6781\u5927\u7684\u4fbf\u5229\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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[1] S. P. Pasilis et al. Anal. Bioanal. Chem. 391 (2008) 317<\/em><\/h6>
[2] S. P. Pasilis et al. J. Mass Spectrom. 43 (2008) 1627<\/em><\/h6><\/section><\/section><\/section>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u4ece\u4ee5\u4e0a\u4f8b\u5b50\u53ef\u89c1\uff0c\u9ad8\u6548\u8584\u5c42\u8272\u8c31\u6cd5\uff0c\u51ed\u7740\u9ad8\u6548\u3001\u5feb\u901f\u3001\u64cd\u4f5c\u7b80\u5355\u3001\u7ed3\u679c\u51c6\u786e\u3001\u7075\u654f\u5ea6\u9ad8\u548c\u91cd\u73b0\u6027\u597d\u7684\u4f18\u52bf\uff0c\u4e0d\u5355\u4e3a\u6837\u54c1\u68c0\u6d4b\u5e26\u6765\u521b\u65b0\u6807\u51c6\uff0c\u52a0\u4e0a\u8fd1\u5e74\u6765\u53d1\u5c55\u8fc5\u901f\uff0c\u914d\u5408\u81ea\u52a8\u5316\u4e0e\u6570\u5b57\u5316\u6280\u672f\uff0c\u66f4\u80fd\u53d1\u6325\u524d\u6240\u672a\u6709\u7684\u7206\u53d1\u529b\uff0c\u6df1\u53d7\u4e1a\u754c\u6b22\u8fce\u3002<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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CAMAG HPTLC PRO \u5168\u81ea\u52a8\u9ad8\u6548\u8584\u5c42\u68c0\u6d4b\u7cfb\u7edf<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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2023 \u5e74 5 \u6708 6 \u65e5<\/strong><\/p>

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\u4e0b\u5348 4:55<\/strong><\/p>

\u5206\u4f1a\u573a 1<\/h6><\/section><\/section>

\u8272\u8c31\u57fa\u7840\u548c\u65b0\u6280\u672f<\/strong><\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

\u8fd1\u5e74\u6765\uff0c\u968f\u7740\u8584\u5c42\u677f\u56fa\u5b9a\u76f8\u6d82\u5c42\u591a\u6837\u5316\u7684\u53d1\u5c55\uff0c\u9ad8\u6548\u8584\u5c42\u8272\u8c31\u6280\u672f\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u8bf8\u591a\u9886\u57df\uff0c\u5c24\u5176\u662f TLC-MS \u7684\u8054\u7528\u548c\u5546\u4e1a\u5316\u8bbe\u5907\u7684\u65e5\u8d8b\u6210\u719f\uff0c\u4e3a\u751f\u7269\u5206\u6790\u7814\u7a76\u63d0\u4f9b\u4e86\u65b0\u7684\u601d\u8def\u3002 \u7814\u7a76\u76ee\u7684 \u672c\u7814\u7a76\u5c06\u808c\u7ea2\u86cb\u767d\u3001\u7ec6\u80de\u8272\u7d20 C\u3001\u03b2-\u916a\u86cb\u767d\u548c\u725b\u8840\u6e05\u86cb\u767d (BSA) \u7b49\u86cb\u767d\u8d28\u7684\u80f0\u86cb\u767d\u9176\u5206\u89e3\u7269\u4e2d\u7684\u80bd\u901a\u8fc7\u9ad8\u6548\u8584\u5c42\u8272\u8c31\u6cd5\u8fdb\u884c\u5206\u79bb\uff0c\u7136\u540e\u901a\u8fc7\u4e0e ESI-MS \u5076\u8054\u5728\u8584\u5c42\u677f\u4e0a\u539f\u4f4d\u5206\u6790\uff0c\u786e\u5b9a\u5e8f\u5217\u8986\u76d6\u7387\u5e76\u4e0e\u6db2\u76f8\u68c0\u6d4b\u7ed3\u679c\u8fdb\u884c\u6bd4\u8f83\u3002 \u7814\u7a76\u65b9\u6cd5\u53ca\u5206\u6790\u7ed3\u679c \u4f7f\u7528\u4e00\u7ef4 HPTLC \u6700\u591a\u5206\u79bb\u5f97\u5230 20 \u4e2a\u6761\u5e26\uff0c\u83b7\u5f97\u975e\u5e38\u7d27\u51d1\u7684\u8584\u5c42\u8272\u8c31\u533a\uff0c\u8fd9\u5927\u7ea6\u662f\u901a\u8fc7\u80f0\u86cb\u767d\u9176\u6d88\u5316\u86cb\u767d\u8d28\u6837\u54c1\u83b7\u5f97\u7684\u80bd\u7684\u6570\u91cf (\u4f8b\u5982\u7ec6\u80de\u8272\u7d20 C \u7531 104 \u4e2a\u6c28\u57fa\u9178\u7ec4\u6210\uff0c\u7ecf\u8fc7\u80f0\u86cb\u767d\u9176\u6d88\u5316\u540e\u5f62\u6210 17 \u4e2a\u80bd\uff09\u3002 HPTLC \u4e00\u7ef4\u5206\u79bb\u8272\u8c31\u56fe\uff1a \u56fe 1. \u8367\u5149\u80fa\u663e\u8272-366nm\uff1b\u56fe 2. \u831a\u4e09\u916e\u663e\u8272-\u767d\u5149 [\u6837\u54c1\u4ece\u5de6\u81f3\u53f3\u5206\u522b\u4e3a\uff1a\u808c\u7ea2\u86cb\u767d\u3001\u7ec6\u80de\u8272\u7d20 C\u3001\u03b2-\u916a\u86cb\u767d\u548c\u725b\u8840\u6e05\u86cb\u767d (BSA)] \u518d\u8fdb\u4e00\u6b65\u8584\u5c42\u5c55\u5f00\uff0c\u901a\u8fc7 HPTLC \u4e8c\u7ef4\u5206\u79bb\uff0c\u53ef\u4ee5\u589e\u52a0\u88ab\u5206\u79bb\u7269\u8d28\u7684\u6570\u91cf\u3002\u7531\u4e0b\u56fe\u53ef\u77e5\u4e00\u7ef4\u5206\u79bb\u8272\u8c31\u53ef\u89c1 11 \u4e2a\u5206\u79bb\u6761\u5e26\uff0c\u4e8c\u7ef4\u5206\u79bb\u8272\u8c31\u53ef\u89c1 17 \u4e2a\u5206\u79bb\u6591\u70b9\u3002 \u56fe 3. HPTLC \u4e8c\u7ef4\u5206\u79bb\u8272\u8c31\u56fe\uff1a\u7ec6\u80de\u8272\u7d20 C \u8868 1. HPTLC \/ DESI-MS \u5206\u6790\u540e\u83b7\u5f97\u7684\u7ec6\u80de\u8272\u7d20 C \u7684\u5e8f\u5217\u8986\u76d6\u7387 \u5e8f\u5217\u8986\u76d6\u7387\u56e0\u56fa\u5b9a\u76f8\u6d82\u5c42\u6750\u6599\u548c\u5206\u79bb\u8d28\u91cf\u800c\u5f02\u3002\u5e94\u7528\u7ea4\u7ef4\u7d20\u677f\u7684\u4e8c\u7ef4\u5206\u79bb\uff0c\u5e8f\u5217\u8986\u76d6\u7387\u51e0\u4e4e\u4e0e HPLC […]<\/p>\n","protected":false},"author":17,"featured_media":17457,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[151,331],"tags":[97,424],"_links":{"self":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts\/17446"}],"collection":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/comments?post=17446"}],"version-history":[{"count":18,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts\/17446\/revisions"}],"predecessor-version":[{"id":17984,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/posts\/17446\/revisions\/17984"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/media\/17457"}],"wp:attachment":[{"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/media?parent=17446"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/categories?post=17446"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nikyang.com\/wp-json\/wp\/v2\/tags?post=17446"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}